中国美容医学2012，Vol.21Issue(17)：2194-2196,3.
骨髓间充质干细胞膜片复合珊瑚支架构建管状组织工程骨的实验研究
Engineering tubular bone constructs using bone marrow mesenchymal stem cells sheets and natural coral
摘要
Abstract
Objective In this study,mesenchymal stem cells (MSCs) sheets were assembled with tubular scaffolds made from natural coral (NA), for engineering of functional bone grafts. Methods MSCs were cultured to form a thin layer of cell sheet via osteogenic induction.Then.tubular long bones were constructed by wrapping the MSCs sheets on to scaffolds.In vivo, constructs were implanted subcutaneously in rabbits. Coral implants were acted as controls.The specimens were harvested and evaluated by gross examination,scanning electronic microscopy (SEM) analysis and histological examination 4 and 8 weeks after implantation. Results In vivo, SEM and histological examinations revealed neo trabecular bone were formed within the constructs in test group. In contrast, the NA was filled only with fibrous tissue in the control group.without evidence of bone formation. Conclusion These findings illustrate that large bone tissues similar to native bone can be regenerated utilizing sheet techniques in conjunction with NA.关键词
骨髓间充质干细胞/骨组织工程/细胞膜片/珊瑚Key words
bone marrow mesenchymal stem cells/ bone tissue engineering/ cell sheet/ natural coral分类
生物科学引用本文复制引用
张蓉,高瞻,雷权,王荣耀,郭增良,李猛..骨髓间充质干细胞膜片复合珊瑚支架构建管状组织工程骨的实验研究[J].中国美容医学,2012,21(17):2194-2196,3.基金项目
新疆维吾尔自治区自然科学基金项目 (2012211A040) （2012211A040）
