中国免疫学杂志2012，Vol.28Issue(11)：963-967,5.DOI:10.3969/j.issn.1000-484X.2012.11.001
IFITM3基因的克隆及其在真核细胞中的表达与定位研究
Cloning of IFITM3 gene and its expression, location in 293T eukaryotic cells
摘要
Abstract
Objective:To obtain the IFITM3 gene by human embryonic kidney cells 293 T induced with interferon rhIFN-α 2B in vitro and observe the IFITM3 gene expression in the transfected 293T. Methods: Human embryonic kidney cells 293T were induced with interferon rhIFN-α 2B in vitro. The complete cDNA of IFITM3 gene was amplified using RT-PCR; and the product was ligased to the pMD18-T vector. The recombinant plasmid pV-IFITM3 was constructed by subcloning the IFITM3 gene into the eukaryotic expression vector pVAX1 after sequencing. Research on expression and location of IFITM3 gene was conducted by tracfecting 293T cells. Results : The results shows that target gene could be expressed and had antigenic activity through RT -PCR and Western blot identification. The IFITM3 gene was mainly located in the outer membrane of 293 T cells observed by Laser scanning confocal microscope. Conclusion; The IFITM3 gene was obtained successfully and its expression was verified . This research provides valuable reference for the fur -ther study of IFITM3 related antiviral drugs and the mechanism of its anti -virus.关键词
IFITM3/克隆/表达/定位Key words
IFITM3/ Clone/ Expression/ Location分类
生物科学引用本文复制引用
朱娜,田宇飞,金宁一,李昌,郭焱,李沂,孙丹丹,任静强,杜寿文,秦艳青,王茂鹏..IFITM3基因的克隆及其在真核细胞中的表达与定位研究[J].中国免疫学杂志,2012,28(11):963-967,5.基金项目
本文受国家自然科学基金(81001342)、 973计划(2011CB512110)和吉林省自然科学基金(201015116)项目资助 （81001342）
