中国免疫学杂志2012，Vol.28Issue(11)：979-985,7.DOI:10.3969/j.issn.1000-484X.2012.11.004
p38MAPK介导的Fas/FasL凋亡信号通路在大鼠抗GBM肾炎中的作用
Effects of apoptosis induced by p38MAPK through Fas/FasL pathway on anti-glomerular basement membrane nephritis in rats
摘要
Abstract
Objective:To investigate the effects of Fas/FasL pathway induced by p38MAPK on anti-glomerular basement membrane (GBM) nephritis in rats. Methods;1: Reproduced the anti-GBM nephritis model. The kidney specimens were collected on the 2nd day, the 7th day, the 14th day, the 21st day and the 28th day and the protein expression of p-p38MAPK,p38MAPK, FasL, Fas, Caspase-3 were detected by Western blot. 2: The nephritis rats were injected intraperitoneally with SB 203580 ( an inhibitor of p38MAPK). All rats were observed for 24 h urinary protein, serum creatine, serum urea on the 2nd day, the 7th day and the 14th day. On the 14th day, the kidney specimens were collected to observe the pathological changes ; the numbers of apoptotic cells were detected by TUNEL method; the expression of p-p38MAPK, p38MAPK, FasL, Fas and Caspase-3 were detected by Western blot and immunohistochemical method. Results:Results from immunoblotting showed that p-p38MAPK, FasL, Fas and Caspase-3 expression of the nephritis group were increased obviously compared with the normal control group at each time point , but there is no difference of p38MAPK expression between the two groups. On the anti-GBM nephritis model in rats , SB203580 depressed the biochemical indicators; lessened the pathological changes ; inhibited the expression of p-p38MAPK, FasL, Fas and Caspase-3; reduced the numbers of apoptotic cells. Conclusion:The apoptosis induced by p38MAPK through Fas/FasL pathway play important effects on anti-GBM nephritis in rats. The renal pathological changes of anti -GBM nephritis can be lessened by SB203580, which maybe provide experimental basis for clinical control.关键词
抗GBM肾炎/p38MAPK/Fas/FasL/细胞凋亡Key words
Anti-GBM nephritis/ p38MAPK/ Fas/FasL/ Apoptosis分类
医药卫生引用本文复制引用
王晓天,李向阳,秦苏萍,李小翠,尤红娟,汤仁仙..p38MAPK介导的Fas/FasL凋亡信号通路在大鼠抗GBM肾炎中的作用[J].中国免疫学杂志,2012,28(11):979-985,7.基金项目
本文为徐州医学院科研课题(08KJ12) （08KJ12）
