中国免疫学杂志2012，Vol.28Issue(11)：1009-1013,5.DOI:10.3969/j.issn.1000-484X.2012.11.011
强直性脊柱炎患者外周血TCR Vα CDR3谱系基因扫描分析研究
Study on the TCR Vα CDR3 Lineage in peripheral blood by GeneScan in patients with ankylosing spondylitis
摘要
Abstract
Objective: To study T cells lineage polymorphism of TCR Vα CDR3 in peripheral blood of ankylosing spondylitis (AS) patients so as to provide experimental basis to immune pathogenesis research in AS . Methods:T cells TCR Va 34 subfamilies in AS patients PBMC were amplified by RT -PCR method, then TCR Va CDR3 lineages polymorphism were analyzed by immunization scanning spectrum. Results:TCR Va CDR3 scanning spectrum of 10 AS patients all showed abnormal distribution peak , including monoclonal, oligoclonal/oligoclonal trend, skewing peak and irregular abnormal peak type subfamilies . A total of 17 subfamilies appeared that monoclonal proliferation of a single peak in 10 cases patients , the frequency in descending order were : Vαl9 subfamily that monoclonal proliferation of a single peak in three patients ; each Vα32, Va7 and Vαl. 2 showed in 2 patients with a single peak ; following showed a single peak. Conclusion: TCR Vα CDR3 lineage have significant polymorphism and spectrum drift characteristics in AS patients peripheral blood , which further indicate that T cells play an important role in immunological pathogenesis in AS . Mono clonal/oligoclonal expansion of T cells may be autoreactive T cells in the pathogenesis of AS , and this study provides a foundation for further research into the mechanism of AS.关键词
强直性脊柱炎/T细胞受体/Vα亚家族/互补决定区3/免疫扫描谱型分析Key words
Ankylosing spondylitis/T cell receptors/Va subfamiles/Complementarity determining region 3/Immunoscope spectratyping分类
医药卫生引用本文复制引用
魏克娜,余伍忠,徐新玉,张璐,邹红云..强直性脊柱炎患者外周血TCR Vα CDR3谱系基因扫描分析研究[J].中国免疫学杂志,2012,28(11):1009-1013,5.基金项目
本文受国家自然科学基金(81172840)和兰州军区医药卫生科研基金资助项目(CWS10JA21)经费资助 （81172840）
