中国脑血管病杂志2012，Vol.9Issue(11)：590-594,5.DOI:10.3969/j.issn.1672-5921.2012.11.008
脑络欣通对局灶性脑缺血 - 再灌注大鼠血液流变学及相关调节因子的作用
Effects of naoluoxintong on hemorheology and related regulatory factors in focal cerebral ischemia-reperfusion rats
摘要
Abstract
Objective To observe the effects of Naoluoxintong on hemorheology (rCBF) and related regulatory factors in rats with middle cerebral artery occlusion-reperfusion (MCAO/R) . Methods Sixty Wistar rats were randomly divided into sham operation, model, tongxinluo, naoluoxintong 40 mg/ml, Naoluoxintong 80 mg/ml,and Naoluoxintong 100 mg/ml groups. A rat model of MCAO/R was induced by the in-traluminal suture method. Whole blood viscosity, plasma viscosity and hematocrit (HCT) were detected by an automatic hemorheology analyzer. The content of fibrinogen ( FIB) was detected by a high performance coagulation analyzer. The levels of thrombopoietin (TPO) and endothelin ( ET) were detected by double antibody sandwich enzyme-linked immunosorbent assay ( ELISA). The rCBF of the rats was detected by laser Doppler flowmetry at the 14th day after modeling in each group. Results ①Whole blood viscosity, plasma viscosity, HCT, and FIB in the sham operation, Tongxinluo and Naoluoxintong groups were all lower than those in the model group. The differences were statistically significant (P < 0. 01 ). ②HCT in the Tongxinluo, Naoluoxintong 80 mg/ml and Naoluoxintong 100 mg/ml groups was lower than that in the Naoluoxintong 40 mg/ml group. The differences were statistically significant (P < 0. 01). ③ The levels of TPO and ET in the sham operation, Tongxinluo and 3 Naoluoxintong groups were lower than those in the model group,but the rCBF was higher than that in the model group. The differences were statistically significant (P < 0. 01 or P < 0. 05 ). ④The level of TPO in the naoluoxintong 80 mg/ml group was lower than that in the naoluoxintong 40 mg/ml group, but the rCBF was higher than that in the naoluoxintong 40 mg/ml group. The difference was statistically significant (P<0. 05). Conclusion Naoluoxintong can improve the hemorheology indexes and regulate the relevant factors of the fibrinolysis coagulation system in MCAO model rats,which has a good effect on ischemic eerebrovascular disease.关键词
血液流变学/血小板生成素/脑络欣通/内皮素/大鼠Key words
Hemorheology/ Thrombopoietin/ Naoluoxintong/Endothelin/ Rats引用本文复制引用
王键,谭辉,菅威,胡建鹏,何玲,李祥..脑络欣通对局灶性脑缺血 - 再灌注大鼠血液流变学及相关调节因子的作用[J].中国脑血管病杂志,2012,9(11):590-594,5.基金项目
国家科技支撑计划(2012BAI26B00) （2012BAI26B00）
安徽省科技厅资助项目(100432) （100432）
康缘中医药科技创新基金项目(KYCX201010) （KYCX201010）
