中国农业科学2012，Vol.45Issue(17)：3515-3521,7.DOI:10.3864/j.issn.0578-1752.2012.17.007
中国红麻炭疽病病原菌的分离鉴定及rDNA-ITS序列分析
Identification and rDNA-ITS Sequence Analysis of the Pathogen of Kenaf Anthracnose
摘要
Abstract
[Objective] The objective of this study is to determine the possible pathogens of kenaf anthracnose of China. [Method] The samples of diseased kenaf were collected and isolated from Anhui, Henan, Zhejiang, Fujian Provinces of China. AH, HN, ZJ, FJ strains were selected from sixteen pathogens and their pathogenicities were determined. Identification was carried out by using traditional morphological methods and sequencing of rDNA-ITS. [Result] The differences among AH, HN, ZJ and FJ strains in morphological characteristics and pathogenicities were relatively abundant. The rDNA-ITS length of AH, HN, ZJ, and FJ strains was 541, 545, 541, and 535 bp, respectively. The homology between FJ strain and other strains was 90%-91%, and the homology among AH, HN, ZJ strains was 96%-98%. [Conclusion] The pathogens of kenaf anthracnose were identified by analysis at the molecular level. It is confirmed that kenaf anthracnose in China is caused by C. dematium and C. gioeosporioides, and the pathogenicity of C. dematium is greater than C. gioeosporioides.关键词
红麻/炭疽病菌/病原种类/致病力/rDNA-ITS序列Key words
kenaf/Colletotrichum/pathogen species/pathogenicity/rDNA-ITS sequence引用本文复制引用
刘晓倩,祁建民,陈玉森,陈绵才,陈美霞,刘伟,方平平,林荔辉,陶爱芬..中国红麻炭疽病病原菌的分离鉴定及rDNA-ITS序列分析[J].中国农业科学,2012,45(17):3515-3521,7.基金项目
国家麻类产业技术体系建设项目(nycytx-19-512)、农业部东南黄/红麻科学观测实验站建设项目(农科教(2011)9号)、福建省南方麻类种质资源共享平台项目(2011N2002)、福建省农业五新项目(Scz10007) （nycytx-19-512）
