中国农业科学2012，Vol.45Issue(19)：3899-3908,10.DOI:10.3864/j.issn.0578-1752.2012.19.001
水稻转基因种质IRBB13(PXa13∶GFP)感病及育性突变体的筛选及鉴定
Screening and Identification of Susceptibility and Fertility Related Mutants from Transgenic Rice IRBB13 (PXa13∶GFP)
摘要
Abstract
[Objective] The objective of this study is to establish a simple and efficient mode of rice mutants selection, and lay the first stone for cloning rice genes involved in regulating xal3/ Xal3 gene expression or their mediated disease resistant /susceptible and pollen development signal pathway. [Method] Using EMS treatment, a rice mutation population was constructed with IRBB13 (PXa13:GFP) which carrying green fluorescent protein(GFP) derived with dominant Xal3 gene promoter. Then the mutants were screened out with the following three periods: At seedling stage, selecting mutants in which GFP gene expression decline either in root or bud or non-expression; At adult stage, selecting mutants restored susceptible to PXO99; At booting stage, selecting mutants reduced fertility. [Result] In total 3 000 M2 lines, 14 and 9 mutants were identified for both root and bud fluorescent declined and fluorescent normal only in root at seedling stage. At adult stage, relying on phenotype of PXO99 inoculation, a total of 25 lines were characterized to restore the susceptibility to PXO99. At booting stage, 7 mutants were identified, of which fertility declined and the green fluorescence of the anthers also disappeared and one mutant was found to restore susceptibility. [Conclusion] A simple and efficient mode of rice mutants selection system was established. Combining the abnormal expression pattern of GFP mutants, restored susceptible phenotype mutants and reduced fertility mutants, it was preliminarily indicated that the two signal transduction pathways mediated by xali/ Xa13 genes are not only completely independent but also partially crossing between resistance/susceptibility and pollen development. Furthermore, after analysis and cloned the genes from these mutants, they will help us better understanding the functional mechanism of xa13/Xa13 genes.关键词
水稻/EMS突变体/绿色荧光蛋白/花粉发育/水稻白叶枯病Key words
rice/EMS mutants/GFP/pollen development/bacterial blight引用本文复制引用
韩宽德,孔令广,巨延虎,陈明,曹志刚,丁新华,储昭辉..水稻转基因种质IRBB13(PXa13∶GFP)感病及育性突变体的筛选及鉴定[J].中国农业科学,2012,45(19):3899-3908,10.基金项目
国家自然科学基金(31071381,30900050)、全国优秀博士学位论文作者专项资金(201027) （31071381,30900050）
