中国农业科学2012，Vol.45Issue(19)：3955-3964,10.DOI:10.3864/j.issn.0578-1752.2012.19.006
连作大豆根际微生物群落结构及土壤酶活性
Effects of Soybean Continuous Cropping on Microbial and Soil Enzymes in Soybean Rhizosphere
摘要
Abstract
[Objective] The objective of the experiment was to determine the effects of continuous and alternate cropping on microbe community structures and soil enzyme activities in soybean, and to provide a scientific basis for the obstacles of continuous cropping of soybean. [Method] Rhizosphere soils of different growth stages was used to characterize the community structure of soil microbe based on phospholipid fatty acid (PLFA) method, and the contents of soil microbial biomass carbon and enzyme activities were determined. [Result] In the soils of alternate and continuous cropping, the total PLFAs biomass, the microbial biomass carbon contents, the activities of urease and invertase all decreased, and the ratios of fungi and bacteria were enhanced, and the activities of catalase had no significant difference among the treatments. There were different influences on microbial and soil enzymes activities in different years of continuous cropping and growth stage, which closely related with the number and type of root exudates and straw decomposing matter. The correlation analysis showed that there was a positive relationship significantly between the total PLFAs biomass and microbial biomass carbon content and the activity of soil urease. There was no relationship between the total PLFAs biomass and the activity of soil catalase and invertase. [Conclusion] The microbial community structures and enzyme activities were changed by continuous and alternate soybean. The obstacles of continuous soybean were the result of the comprehensive effect of soil micro-ecological system and environmental factor.关键词
大豆/磷脂脂肪酸/连作障碍/微生物群落结构Key words
soybean/phospholipid fatty acid/continuous cropping/community structure引用本文复制引用
谷岩,邱强,王振民,陈喜凤,吴春胜..连作大豆根际微生物群落结构及土壤酶活性[J].中国农业科学,2012,45(19):3955-3964,10.基金项目
国家自然科学基金青年基金(31000687)、吉林农业大学校内博士启动基金 （31000687）
