中国农业科学2012，Vol.45Issue(19)：4075-4083,9.DOI:10.3864/j.issn.0578-1752.2012.19.020
母猪初情期全基因组关联分析和位置候选基因研究
Genome-Wide Association Study and Positional Candidate Gene Analysis on Age at Puberty of Gilts
摘要
Abstract
[Objective] Isolation and characterization of the causative gene or molecular marker for age at puberty will contribute to the selection and breeding of sows. [Method] The materials used in this study were F2 gilts from a white Duroc x Erhualian F2 resource population. F0, F1 and 316 well phenotyped F2 gilts were genotyped with Illumina porcine 60K. SNP chip. Genome-wide association study (GWAS) and linkage and linkage-disequilibrium analysis (LDLA) were carried out to detect SNPs or haplotypes that were significantly associated with age at puberty of gilts. The associations of 3 SNPs within two candidate genes of Lin-28 Homolog B (LIN28B) and Trans-membrane Protein 38B (TMEM38B) with age at puberty of gilts were evaluated by standard association test, marker-assisted association test and F-drop test. [Result] The results from GWAS showed that the ASGA0032316 was the most significant SNP that was associated with age at puberty. It is located at 33.07 Mb of pig chromosome 7 (SSC7) and exactly within the intron region of RAB23. At the same instant, several significant SNPs were also detected on chromosomes 1,6, 12, 15 and 17. The results from LDLA analysis showed that all haplotypes that were associated with age at puberty at genome-wide significance level were located on SSC7. The most significant haplotype was located at 38.39-38.47 Mb and within the intergenic region of F1RVR7 and ZFAND3. In association analyses of positional candidate genes, although 3 SNPs from LIN28B and TMEM38B were significantly associated with age at puberty in standard association test (P<0.05), it was not achieved significance level both in the marker-assisted association test and F-drop test (P>0.05). [Conclusion] The SNP and haplotype that were most significantly associated with age at puberty of gilts was detected on SSC7. Several other significantly associated SNPs were also detected on chromosomes 1,6, 12, 15 and 17. The results from association tests suggested that LIN28B or TMEM38B gene on SSC1 is not the quantitative trait gene for age at puberty, or more SNPs from two genes need to be further analyzed.关键词
母猪初情期/全基因组关联分析/连锁和连锁不平衡分析/位置候选基因Key words
age at puberty of gilts/genome-wide association study/linkage and linkage-disequilibrium analysis/positional candidate gene引用本文复制引用
李平华,李杰,杨竹青,张志燕,杨斌,陈从英..母猪初情期全基因组关联分析和位置候选基因研究[J].中国农业科学,2012,45(19):4075-4083,9.基金项目
江西省自然科学基金(20114BAB204002) （20114BAB204002）
