中国农业科学2012，Vol.45Issue(19)：4092-4101,10.DOI:10.3864/j.issn.0578-1752.2012.19.022
内蒙古白绒山羊褪黑激素受体基因SNP与产绒性状的关联
Correlations Between SNP of MTNR Gene and Cashmere-Producing Traits of Inner Mongolia White Cashmere Goat
摘要
Abstract
[Objective] Correlations between SNP of cashmere goat melatonin receptor (MTNR) gene and cashmere-producing traits were studied to find molecular marker of MAS for molecular breeding. [Method] Arbas white cashmere goats and partial sequences of MTNRla and lb genes were used as experimental samples and candidate genes, respectively. PCR-SSCP and DNA sequencing technologies were conducted to detect their single nucleotide polymorphism. Results were analyzed by least square mean (LSM) among production traits of genotypes. [Result] There was no SSCP in the exons 1 and 2 of MTNRla gene and the exon 1 of MTNRlb gene, while there was the SSCP in the exon 2 of MTNRlb gene and three of SNP were detected. P4 primer loci existed in C-G mutation of NM_001206907:g.648 bp which didn't result in change of amino acid, and G-A mutation of NM_001206907:g.665 bp which led to CGC-CAC of the 223rd codon from Arg into His. The results showed that polymorphism loci had an significant effect on cashmere yield (P<0.05), and cashmere yield of AA genotype increased by 98.78 g and 148.23 g, as compared to AB and BB, respectively. Meanwhile, there was a significant correlation (P<0.05) in interaction effect between the polymorphism loci genotype and age cashmere thickness. P5 primer loci existed in G-A mutation of NM001206 907:g.l015 bp which led to GGC-AGC of the 339th codon from Gly into Ser. The analyses indicated that interaction effect of the polymorphism loci genotype and age was the marginal significant correlation (P<0.1) with cashmere Fineness. P4 and P5 primes locus were not significantly (P >0.05) with kidding number and birth weight. [Conclusion] P4-AA genotype can be used as molecular marker of MAS for cashmere-producing traits of Inner Mongolian Arbas white cashmere goats.关键词
绒山羊/褪黑激素受体基因/单核苷酸多态性/产绒性状Key words
cashmere goat/MTNR gene/SNP/cashmere-producing traits引用本文复制引用
刘斌,李金泉,赵存发,李玉荣,高凤芹,高娃,成立新,孟克巴雅尔,马跃军,刘少卿..内蒙古白绒山羊褪黑激素受体基因SNP与产绒性状的关联[J].中国农业科学,2012,45(19):4092-4101,10.基金项目
国家绒毛用羊产业技术体系放牧营养调控岗位(CARS-40-12)、内蒙古自治区科技计划项目(20110718)、内蒙古农牧业科学院青年创新基金项目(2009QNJJM04) （CARS-40-12）
