中国人兽共患病学报2012，Vol.28Issue(12)：1185-1190,6.DOI:10.3969/cjz.j.issn.1002-2694.2012.12.006
羊口疮病毒F1L蛋白二级结构分析与表位预测
Prediction of secondary structures and cell epitopes of F1L protein of ORFV
摘要
Abstract
The network platform SOPM A server was used to predict the secondary structure of ORFV F 1L protein . On the basis of the DNAStar prediction results (hydrophilicity , accessibility , flexibility and antigenicity ) , the B cell epitopes of ORFV F1L protein was predicted by screening the secondary structure and followed by verification using ABC prod program . The cytotoxic T cell (CTL) epitopes of ORFV F1L protein were predicted by combination of quantitative matrix (QM ) and artificial neural network (ANN) . T helper (Th) epitopes of ORFV F1L protein were predicted by an online server for the prediction of M HC class II-peptide binding affinity . The result showed that ORFV F1L protein had alpha helixes (34 .71% ) , random coils (40.59% ), β-sheet (18.82% ) and β-turn (5.88% ). No signal peptide was found, while 7 B-cell epitopes, 2 CTL epitopes and 3 Th epitopes of ORFV F1L protein were predicted . These findings would provide theoretical basis for establishment of ORFV diagnosis method , preparation of monoclonal antibody and development of vaccine .关键词
羊口疮病毒/F1L基因/二级结构/细胞表位/预测Key words
ORFV / F1L protein/ secondary structure/ cell epitope / prediction分类
医药卫生引用本文复制引用
王光祥,尚佑军,吕占禄,张克山,田宏,刘湘涛..羊口疮病毒F1L蛋白二级结构分析与表位预测[J].中国人兽共患病学报,2012,28(12):1185-1190,6.基金项目
国家现代肉羊产业技术体系(CARS-39) （CARS-39）
