中国输血杂志2012，Vol.25Issue(9)：834-836,3.
悬浮红细胞储存时间对制备的洗涤红细胞钾离子和游离血红蛋白含量的影响
Influence of the storage time of red blood cells on the concentrations of [K +] and FHb of the prepared washed red blood cells
摘要
Abstract
Objective To study the influence of the storage time of red blood cells (RfiCs) on the concentrations of potassium ( [ K + ] ) and free hemoglobin ( FHb) of prepared washed red blood cells ( washed RBCs). Methods RBCs preserved for 3 d (3 d group,n =6) ,16 d (16 d group,n =6) and 31 ~35 d (31 ~35 d group,n =7) were chosen to prepare washed RBCs manually,and samples were taken at the time before preparation,0,16 and 24 h after preparation, respectively. [K+ ] and FHb were measured. Results The [K+ ] of washed RBCs 24 h after preparation of 3 d group,16 d group and 31 ~35 d group were (3. 26 ±0.57), (3. 39 ±1.12) and (2.97 ±0. 95) mmol/L, respectively (P > 0. 05 ), and all the [ K+ ] were still lower than the upper limit of [ K+ ] in normal human bodies. The FHb of washed RBCs 24 h after preparation of these three groups were (0.29 ±0.18) , (0. 63 ±0.40) and (1.06 ±0. 55) g/L, respectively. The FHb of washed RBCs 24 h after preparation of 31 - 35 d group was significantly higher than that of 3 d group ( P < 0. 01 ) . Conclusion There is little influence of the storage time of RBCs on the [ K+ ] of prepared washed RBCs, with the [ K+ ] of all prepared washed RBCs lower than the upper limit of [ K+ ] in normal human bodies. However, the storage time of RBCs affects the FHb of prepared washed RBCs significantly, so RBCs close to the end of storage should be avoided to prepare washed RBCs.关键词
悬浮红细胞/储存时间/洗涤红细胞/钾离子浓度/游离血红蛋白Key words
Red blood cells/ Storage time/ Washed red blood cells/Concentrations of potassium/ Free hemoglobin分类
临床医学引用本文复制引用
冯博,段澜,王景文,任芙蓉..悬浮红细胞储存时间对制备的洗涤红细胞钾离子和游离血红蛋白含量的影响[J].中国输血杂志,2012,25(9):834-836,3.基金项目
卫生部卫生行业科研专项(201002005) （201002005）
