中国输血杂志Issue(10)：990-994,5.
全血制备浓缩血小板的汇集及滤除白细胞的多中心研究
A Multicenter study on preparation of Leukodepleted Platelet Concentrates from Pooled whole blood-derived platelets
摘要
Abstract
Objective To cooperate with a number of blood centers and evaluate the quality of pooled platelet and leukocyte depletion, which aims to supply basis for establishing operating procedures and quality standard of pooled platelet and leukocyte depletion. Methods PCs was prepared from 400 ml fresh whole blood by platelet-rich plasma (PRP) or buffy-coat (BC) method. 10 to 16 units of ABO-matched PCs were pooled, and then filtered with two types of domestic filters (namely group A and B) randomly. The conventional and functional indicators of platelet were evaluated before and after filtering, and the total samples were 202. Results The total of cases that number of platelet was more than 2. 4 × 1011 was 147, including 77 cases in group A and 70 cases in group B. In group A the number of platelet and leukocyte before and after filtering were (2. 90 ±0.45) xlO" 75(2.60±0.43) × 1011 ,(176.45 ±135.67) × 106 VS( 1.00 ±2.29) ×106 respectively ,and in group B the number of platelet and leukocyte before and after filtering were(2. 80 ±0. 36) × 1011 VS(2. 40 ± 0.37) × 1011, (175. 76 ±147. 84) × 106 VS(0. 30 ±0. 72) × 106 respectively. While the total of cases that number of platelet was less than 2. 4 × 1011 was 55, including 29 cases in group A and 26 cases in group B. In group A the number of platelet and leukocyte before and after filtering were (1. 71 ±0.39)×1011 VS( 1.43 ±0.42) × 1011 ,(65. 85 ±110. 97) ×106 VS (3. 7 ±3. 98) × 106 respectively,and in group B the number of platelet and leukocyte before and after filtering were(l. 79 ±0.48) ×1011 VS(1.42±0.46) × 1011 ,(70. 63 ±145. 55) × 106 VS(1. 45 ±2. 66) × 106 respectively. There were no significant difference(P>0. 05) of these indicators such as pH value,hypotonic shock response (HSR) ,CD62p expression ( % ) and platelet aggregation after filtering in group A and B. Conclusion Pooling and filtering platelet concentrates prepared by PRP and BC method can remove leukocyte effectively and there is no significant change of platelet function after filtering,which meets the requirement of relevant standards. This study supplies basis for establishing operating procedures and quality standard of preparation of leukodepleted platelet concentrates from pooled whole blood-derived platelets.关键词
浓缩血小板/汇集/滤除白细胞/多中心研究Key words
Whole blood-derived platelets/Pool/Leukodepletion/Muiticenter study分类
医药卫生引用本文复制引用
王红,吴瑕,钟锐,贺曾,曹晔,何语良,陈洁,刘嘉馨..全血制备浓缩血小板的汇集及滤除白细胞的多中心研究[J].中国输血杂志,2012,(10):990-994,5.基金项目
国家卫生公益性行业科研专项(编号:200902008) （编号:200902008）
