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核酸检测中HIV病毒载量对内质控扩增的影响

余谨 毕昊 陆华新 王先广 赵磊 沈钢

中国输血杂志Issue(10):1005-1007,3.
中国输血杂志Issue(10):1005-1007,3.

核酸检测中HIV病毒载量对内质控扩增的影响

Effects of HIV viral load on internal control amplification by using NAT technique

余谨 1毕昊 1陆华新 1王先广 1赵磊 1沈钢1

作者信息

  • 1. 武汉血液中心,湖北武汉430033
  • 折叠

摘要

Abstract

Objective To determine the prevalence of viral infections (HBV.HCV and HIV) in serological window period in blood donors screened with nucleic acid testing (NAT) . Methods All blood donors were tested with serological tests( Ag-HBVs,Anti-HCV and Anti-HIV)and molecular testing with NAT by PP6 and rPPl for HBV.HCV and HIV. The window period was defined with the positive NAT and negative serological test. Results During ten months, we evaluated 16 769 blood donors. One subject was identified with HIV in serological window period. HIV viral load has the effect on internal control amplification by using NAT technique. Conclusion Unsafe blood in the performance of viral infections due to HIV in serological window period in donors by using NAT technique.

关键词

核酸检测(NAT)/病毒载量/HIV

Key words

Nucleic acid testing(NAT)/ viral load/ HIV

分类

医药卫生

引用本文复制引用

余谨,毕昊,陆华新,王先广,赵磊,沈钢..核酸检测中HIV病毒载量对内质控扩增的影响[J].中国输血杂志,2012,(10):1005-1007,3.

中国输血杂志

OA北大核心CSCDCSTPCD

1004-549X

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