中国实验动物学报2012，Vol.20Issue(6)：5-9,后插2,6.DOI:10.3969/j.issn.1005-4847.2012.06.002
青少年食蟹猴糖尿病模型的肝脏病理生理学研究
Pathophysiological changes of the liver in juvenile diabetic cynomolgus monkeys
摘要
Abstract
Objective The purpose of this study was to investigate the mechanisms and characteristics of hypergly -cemia^nduced liver injury in streptozotocin induced juvenile diabetic cynomolgus monkeys with poor glycemic control . Methods A total of eight cynomolgus monkeys (3 years old) were used in this experiment. Four monkeys were induced insulin-de pendent diabetes mellitus for four years . Other four age snatched healthy monkeys were used as the controls . Blood tests, periodic acid-Schiff (PAS) staining, Sudan III staining, Gordon-Sweet staining, Masson trichrome staining, conventional light microscopic and ultrastructural morphometry of the liver tissue were performed in both groups . Results Total bile acid, blood urea nitrogen , alanine aminotransferase , aspartate aminotransferase , cholinesterase , lactate dehydro -genase, total cholesterol, triglycende and low density lipoprotein cholesterol levels were significantly increased in the dia -betic monkeys than in the normal monkeys . Histological examination showed swollen hepatocytes , increased number of pos -itive PAS staining and Sudan III staining cells . Ultrastructural observation indicated that hepatocytes contained a drastic in -crease of glycogen granules and high electron -dense mitochondria, and hepatic stellate cells were also considerably more nu - merous in the diabetic livers. Conclusions Juvenile diabetic cynomolgus monkeys with poorly controlled blood sugar showed specific pathologic changes including excess accumulation of glycogen in hepatocytes and increased number of he -patic stellate cells. These pathologic changes were distinct from nonalcoholic fatty liver disease , however, the mechanisms are unclear yet.关键词
链脲佐菌素/肝脏/食蟹猴/糖尿病Key words
Streptozotocin/Liver/Cynomolgus monkeys/Diabetes mellitus分类
生物科学引用本文复制引用
邹春林,滕夏虹,黄芬,王淑艳,吴迪,张愚..青少年食蟹猴糖尿病模型的肝脏病理生理学研究[J].中国实验动物学报,2012,20(6):5-9,后插2,6.基金项目
国家重大科学研究计划重大科学问题导向项目(2012CBA01307) （2012CBA01307）
广西高等学校一般资助科研项目(201203YB042). （201203YB042）
