中国实验动物学报2012，Vol.20Issue(6)：31-37,7.DOI:10.3969/j.issn.1005-4847.2012.06.007
黄河裸裂尻鱼肥胖基因克隆及其对青藏高原季节性冷环境的适应性表达
Molecular cloning of ob gene in Schizopygopsis pylzovi and its mRNA expression responding to seasonal cold environment at Qinghai-Tibetan Plateau
摘要
Abstract
Objective In order to provide scientific data for understanding the adaptive mechanism of Schizopygopsis pylzovi to seasonal cold environment at Qinghai -Tibetan Plateau, the obese gene (ob) of Schizopygopsis pylzovi was cloned and its encoding protein leptin was analyzed . Then the expression pattern of the ob mRNA in tissues and the relative expression level of the ob mRNA in he patopancreas and white muscle of Schizopygopsis pylzovi from habitats of different tern -peratures were assessed. Methods The fulHength cDNA of ob gene was cloned by RT-PCT, 5'41ACE and 3'41ACE methods. The expression pattern and relative expression level were determined by RT -PCR and reaMime PCR. Results The fulHength cDNA of ob gene was 1 337 bp, including a 513 bp complete ORF encoding a 170 amino acid peptide. The ob gene was expressed in the nine examined organs , including heart, kidney, fat, intestine, spermary, hepatopancreas, gill, brain and muscle, while strongly expressed in heart and weakly expressed in muscle . The ob gene expression in the he pat o pancreas and white muscle of Schizopygopsis pylzovi was suppressed by low temperature. Conclusions The tissue expression pattern of ob gene in Schizopjgopsis pylzovi may be related to energy metabolism and biological functions in spe cific tissues. The encoding protein leptin plays an important role in the regulation of energy metabolism of Schizopjgopsis pylzovi, as well as its adaptation to the seasonal cold environment at Qinghai -Tibetan Plateau.关键词
黄河裸裂尻鱼/肥胖基因/克隆/表达/适应/环境温度Key words
Schizopygopsis pylzovi/Ob gene/Cloning/Expression/Adaptation/Temperature, environmental分类
生物科学引用本文复制引用
赵兰英,祁得林,晁燕,李长忠,谢保胜,申志新,王国杰,王振刚,李超,拜彬强..黄河裸裂尻鱼肥胖基因克隆及其对青藏高原季节性冷环境的适应性表达[J].中国实验动物学报,2012,20(6):31-37,7.基金项目
国家自然科学基金项目(30860048,31160226) （30860048,31160226）
教育部新世纪优秀人才支持计划(NCET-09-0861). （NCET-09-0861）
