中国实验动物学报2012，Vol.20Issue(6)：57-60,4.DOI:10.3969/j.issn.1005-4847.2012.06.012
人胃癌细胞株裸鼠皮下移植瘤模型的建立及其生物学特性
Establishment of a nude mouse model of human gastric cancer andcharacterization of its biological features
摘要
Abstract
Objective To establish a subcutaneous tumor model in nude mice by injection of human gastric car -cinoma cell line BGC-823 cells in various concentrations and observe its biological characteristics . Methods BGC-823 cell line was cultured and the cells were divided into four cell suspension groups ; groupl ;5 X 10 cells/mL, n = 10; group 2 ;1 xl0 7 cells/mL, n = 10; group3:l X 10 6 cells/mL, n = 10; group4:l X 10 5 cells/mL, n =10. Each of 40 mice was subcu-taneously injected 0. 2 ml BGC-823 cell suspension into the armpit. Food intake and activity status of tumor -bearing mice, tumor formation time , mental conditions , survival rate , tumor formation rate , growth and size of the tumors were observed . Immunohistochemical SP staining was adopted for assessment of the microvessel density ( MVD) . Results There was no abnormity and death in the mice after injection of tumor cell suspension . The overall tumor formation rate was 100% in all groups except the group 4,and the average tumor appearing time was 3-7 days after injection of tumor cell suspension . The average tumor MVD was (123. 26 ±31. 57)/mm . Conclusions The subcutaneous nude mouse models of human gastric carcinoma and the lowest concentration of tumor cells needed for the modeling are preliminarily established . It lays a foundation for experimental studies of gastric carcinoma .关键词
胃癌细胞株/裸鼠/肿瘤模型/肿瘤血管密度/裸鼠模型Key words
Human gastric carcinoma/Cell line/Nude mice/Tumor model/Microvessel density, MVD/Nude mouse models分类
生物科学引用本文复制引用
马嵋,张燕,张玲,魏琴,段明军,侯月梅..人胃癌细胞株裸鼠皮下移植瘤模型的建立及其生物学特性[J].中国实验动物学报,2012,20(6):57-60,4.基金项目
新疆医科大学第一附属医院干细胞专项基金(2010GXB03). （2010GXB03）
