中国体外循环杂志2012，Vol.10Issue(4)：232-234,256,4.
氯通道阻断剂NPPB拮抗离体灌注大鼠心脏钙矛盾处理引起的心肌损伤
Chloride channel blocker NPPB blunted heart injury induced by calcium paradox in rat
摘要
Abstract
Objective This study evaluated the effect of chloride channel blocker 5 - nitro - 2 - ( 3 - phenyl - propylamino ) - benzoic acid ( NPPB ) on heart injury induced by calcium paradox. Methods Calcium paradox in isolated rat heart was elicited by 3- min calcium depletion followed by 30 - min calcium repletion. 20 μmol/L NPPB was added 2 min prior to calcium depletion, throughout the period of calcium depletion and the first 5 min alter calcium repletion. Cardiac function was monitored and infarct size measured. Results Comparing to control group, calcium paradox treatment produced a marked depression in cardiac function and induced an enlarged infarct size. In NPPB treatment group, all the 10 hearts showed a marked decrease in infarct size, and the left ventricular developed pressure ( LVDP) rescued in 4 of 10 hearts. Meanwhile, the left ventricular end - diastolic pressure ( LVEDP) and dp/dt were improved in NPPB treatment group, although they did not reach significant difference. NPPB at 20 μmol/L had no effect on cardiac performance and cell death at the end of perfusion under control conditions. Conclusion The results demonstrate that NPPB protects heart against calcium paradox. It also indicates that chloride channels are involved in.关键词
心脏/氯通道/钙离子/大鼠/心肌损伤Key words
Heart/ Chloride channel/ Calcium分类
医药卫生引用本文复制引用
张建英,冯建宇,李雨衡,金振晓,周京军..氯通道阻断剂NPPB拮抗离体灌注大鼠心脏钙矛盾处理引起的心肌损伤[J].中国体外循环杂志,2012,10(4):232-234,256,4.基金项目
国家自然科学基金资助项目(30971196) （30971196）
