中国畜牧兽医2012，Vol.39Issue(11)：31-34,4.
牛副流感病毒3型RT-LAMP检测方法的建立及应用
Establishment and Application of Loop-mediated Isothermal Amplification for Bovine Parainfluenza Virus 3
摘要
Abstract
A set of primers used for reverse transcription loop-mediated isothermal amplification (RT-LAMP) detection was designed based on the conserved nucleoprotein gene of bovine parainfluenza virus 3(BPIV-3) complete genome sequence submitted in GenBank. The usefulness of RT-LAMP for rapid preciinical detection of BPIV-3 infection was evaluated. The reaction could be finished in 1 h under isothermal condition at 63 ℃. This RT-LAMP assay had a detection limit of 0. 069 fg/μL per reaction, was higher sensitivities than that of RT-PCR. The specificity of this assay could be easily confirmed by agarose gel elec-trophoresis, color reaction or turbidity comparison. As a result, the RT-LAMP assay was an ideal method for detecting BPIV-3 in laboratory and primary diagnosis of clinical infection.关键词
牛副流感病毒3型/N基因/反转录-环介导等温扩增Key words
bovine parainfluenza virus type 3/ nucleoprotein gene/ reverse transcription loop-mediated isothermal amplification分类
农业科技引用本文复制引用
师新川,温永俊,王凤雪,胡嘉欣,杨博超,王炜,宋妮,程世鹏,武华..牛副流感病毒3型RT-LAMP检测方法的建立及应用[J].中国畜牧兽医,2012,39(11):31-34,4.基金项目
"863"计划——"新型重大动物疫苗与诊断试剂创制与工艺创新"(2011AA10A213). （2011AA10A213）
