中国畜牧兽医2012，Vol.39Issue(11)：39-42,4.
糖化酶单抗的研制及其抗原识别位点的分析
The Development and Antigenic Analysis of Monoclonal Antibodies to Glucoamylase
摘要
Abstract
To develop a detection method for glucoamylase in honey, BALB/c mice were immunized with the glucoamylase from A.spergillus niger. Splenocytes of the immunized mice were fused with SP2/0 cells with PEG. 12 monoclonal antibodies (McAbs) against the glucoamylase were obtained and identified. 10 hybridoma cell lines represented subtypes IgG1 and the other were IgG2b, and their light chains were κ chain. Western blotting analysis showed that the 12 McAbs specifically recognized glucoamylase. Hybridoma 2H4F9 ,6H9D8 ,8F2F11 .8F2E9,1A8G6 , 1C4D5 excreting anti-glucoamylase McAbs were used to be injected into abdominal cavity of mice(ICR). The titers of the McAbs were all above 1:1×104. Six strains of monoclonal antibodies were confirmed to be specific for four different kinds of epitopes on glucoamylase competitive binding assay. Among them, McAb-6H9D8 and MeAb-8F2F11 might direct to the same antigenic determinant on the GA, McAb-lA8G6 and McAb-1C4D5 to the second antigenic site, McAb-8F2E9 to the third antigenic site, McAb 2H4F9 to the fourth antigenic site.关键词
淀粉糖化酶/单克隆抗体/抗原识别位点Key words
glucoamylase/monoclonal antibodies/epitopes分类
农业科技引用本文复制引用
张丹,姜威,李双喜,汪惠泽,冯云飞,刘云迎,李东明,何祥来,王捍东..糖化酶单抗的研制及其抗原识别位点的分析[J].中国畜牧兽医,2012,39(11):39-42,4.基金项目
江苏高校优势学科建设工程和江苏省高校重点实验室开放课题资助. （）
