中国畜牧兽医2012，Vol.39Issue(11)：56-61,6.
巴马小型猪主要细胞因子的克隆及序列分析
Cloning and Sequence Analysis of the Main Cytokines in Bama Miniature Pig
摘要
Abstract
In order to enrich the cytokine biological data of Guangxi Bama miniature pig, the lymphocytes of Bama miniature pig was separated from peripheral blood and the total RNA was extracted from the peripheral blood lymphocytes, respectively. The target genes of cytokine were amplified by RT-PCR and cloned into pMD18-T vector and transformed into E. Coli DH5a, respectively. We chose positive clones and submitted them to the NCBI after sequencing the recorabinant strain. Comparing homology of the cloned sequence with the known sequence, and using the software to analysis the cloned sequence. The results showed that the gene length of cloned cytokines IL-2, IL-12, IFN-γ, TNF-α of Bama miniature pig were 338, 377, 380, 402 bp,respectively. It was consistent with the expected length of target gene. The sequences analysis showed that the homology of the cloned genes was relatively high with known domestic pigs or wild boars. The sequence homology of IL-2, IL-12, IFN-γ,TNF-α were 98. 9%, 99.5%, 99.2%, 100%, respectively. The research on cytokine gene sequence of Bama miniature pigs not only enriched the biological data of Bama miniature pigs, but also laid the foundation for research of the cytokine function. It also provided the basis for the quantitative detection of cytokine.关键词
巴马小型猪/细胞因子/克隆/序列分析Key words
Bama miniature pig/cytokine/ clone /sequence analysis分类
生物科学引用本文复制引用
李连峰,姜骞,林欢,李红,刘家森,郭东春,原冬伟,崔玉东,曲连东..巴马小型猪主要细胞因子的克隆及序列分析[J].中国畜牧兽医,2012,39(11):56-61,6.基金项目
国际科技合作计划项目(2010DFB33620). （2010DFB33620）
