中国畜牧兽医2012，Vol.39Issue(11)：69-72,4.
牛PU.1基因真核表达载体的构建、表达及鉴定
Construction,Expression and Identification of Eukaryotic Expression Vector of Bovine PU.1 Gene
摘要
Abstract
Total RNA was extracted from bovine,the cDNA encoding PU. 1 protein was cloned using reverse transcription polymerase chain reaction (RT-PCR). The PU. 1 gene was subcloned into pIRES2 DsRed-Express2 vector and then the recom-binant plasmids were transferred into H293T cells and bovine fetal fibroblasts cells by the method of lipofectaming. The results showed that we had successfully constructed the expression plasmid of PU. 1 gene by fluorescence observation and identification of Western blotting, which layed the foundation for the differentiation of bovine blood monocytes and breeding for disease resistance in cattle.关键词
牛/PU.1基因/单核细胞Key words
cattle/PU. 1 gene/monocytes分类
生物科学引用本文复制引用
刘利,张路培,高雪,高会江,李俊雅,许尚忠..牛PU.1基因真核表达载体的构建、表达及鉴定[J].中国畜牧兽医,2012,39(11):69-72,4.基金项目
优质肉牛新品种(系)选育与关键技术研究及示范(2011BAD28B04) （系）
基于高密度SNP建立中国肉牛群体全基因组选择优化方案(2010jc-2). （2010jc-2）
