中国畜牧兽医2012，Vol.39Issue(11)：157-160,4.
冷冻速率和解冻温度对猪精液冷冻效果的影响
Effect of Different Freezing Frequency and Thawing Temperature on Cryopreservation of Boar Semen
摘要
Abstract
For improving the freezing and de-freezing methods for boar semen further, it was aimed to investigate the effect of different freezing frequency( -100 ℃ 10 min, -120 ℃ 10 min, -140 ℃ 10 min) and thawing temperature(37 ℃ 30 s,45 ℃ 30 s,52 ℃ 30 s,60 ℃ 30 s) on cryopreservation of boar semen. The results showed that the sperm motility, plasma integrity, normal acrosome rate by freezing treatment with -120 ℃ 10 min, were all significantly higher than those of the other three groups respectively(P<0. 05). The sperm motility, plasma integrity, normal acrosome rate by thawing treatment with 37 ℃ 30 s, were not significantly higher than those of the other three groups respectively(P>0. 05) , but the difference of the sperra abnormality in the treatment with 60 ℃ 30 s was significant(P<0. 05) , but with 45 ℃ 30 s and 52 ℃ 30 s were not significant (P>0. 05). Therefore, it could be concluded that the combination of freezing by -120 ℃ 10 min and thawing by 37 ℃ 30 s were appropriate to frozen-thawed 0. 25 mL pipettes of boar semen.关键词
猪/精液冷冻/细管/冷冻速率/体外受精Key words
boar/cryopreservation/dtraw/freezing frequency/artificial insemination分类
农业科技引用本文复制引用
梁鸿斌,姚学军,李维华,邵慧,朱金清,邬纯鸿,倪和民,刘云海,郭勇..冷冻速率和解冻温度对猪精液冷冻效果的影响[J].中国畜牧兽医,2012,39(11):157-160,4.基金项目
十一五国家科技支撑计划项目"荣昌猪资源保护体系及关键技术研究"(2007BAD51B01-1-2). （2007BAD51B01-1-2）
