中国畜牧兽医2012，Vol.39Issue(11)：198-200,3.
一例猪伪狂犬病病毒的分离鉴定
The Isolation and Identification of the Porcine Pseudorabies Virus
鲍玉林1
作者信息
- 1. 青海省畜牧兽医职业技术学院,青海湟源 812100
- 折叠
摘要
Abstract
The brain tissue of piglet was obtained and the 217 bp long fragment in gp50 gene was amplified by the polymer-ase chain reaction (PCR). Therefore, the piglet was confirmed as porcine pseudorabies virus infection. Subsequently, the porcine pseudorabies virus was isolated on BHK-21 cells after 5 generations. The cells displayed typical cell lesions and were identified as porcine pseudorabies virus by PCR. The virus infectivety of the pseudorabies virus was 108.68 TCID500/0. 1 Ml by Reed-Muench. Finally, the rabbits were inoculated with 107.0 TCID50/Ml virus. On 48 h the injection sites showed typical itching, skin damage and other symptoms. The rabbits were dead after 72 h by the inoculation of pseudorabies virus. Consequently, the results indicated that the isolated pseudorabies virus was highly pathogenicity to susceptible animals. It would be the research foundation of the virus epidemiology, pathogenesis,vaccine and diagnositic.关键词
猪伪狂犬病病毒/分离鉴定/PCRKey words
porcine pseudorabies virus,isolation and identification/PCR分类
农业科技引用本文复制引用
鲍玉林..一例猪伪狂犬病病毒的分离鉴定[J].中国畜牧兽医,2012,39(11):198-200,3.
