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副猪嗜血杆菌的分离鉴定及药敏试验

迟阳 高慎阳 郭伶 王宏军 董筱萍 周铁忠 胡桂学

中国畜牧兽医2012,Vol.39Issue(11):206-209,4.
中国畜牧兽医2012,Vol.39Issue(11):206-209,4.

副猪嗜血杆菌的分离鉴定及药敏试验

Isolation, Identification and Drug Sensitivity Test of Isolated Haemophilus parasuis

迟阳 1高慎阳 2郭伶 2王宏军 2董筱萍 2周铁忠 2胡桂学1

作者信息

  • 1. 吉林农业大学动物科学技术学院,吉林长春130118
  • 2. 辽宁医学院畜牧兽医学院,辽宁锦州 121001
  • 折叠

摘要

Abstract

One strain of bacteria was isolated from Liaoning samples of pigs with suspected multiple subserosal inflammation and arthritis in 2011. We used the lungs, pericardial fluid, blood from diseased samples via bacterial isolation and culture techniques, biochemisty tests in addition to PCR identification finally indicated that the bacteria belonged to Hemophilus parasuis. Then drug sensitive test and analysis were performed on the strain named HPSLN111013. The results showed that the strain on some aspects such as morpha, cultural character and biochemisty tests were in accord with separate strains from other areas in China. Then drug sensitive test revealed that the isolate was the most sensitive to florfenicol, ceftiofur and lomefloxa-cin. These results supplied a foundation for the future research of Hemophilus parasuis 's serological and gene typing. Ascertaining the infection source and genetic variation of Hemophilus parasuis provided a basis of prevention and control measures of this disease in Liaoning province.

关键词

副猪嗜血杆菌/分离鉴定/药敏试验

Key words

Hemophilus parasuis/ isolation and identification/ drug sensitivity test

分类

农业科技

引用本文复制引用

迟阳,高慎阳,郭伶,王宏军,董筱萍,周铁忠,胡桂学..副猪嗜血杆菌的分离鉴定及药敏试验[J].中国畜牧兽医,2012,39(11):206-209,4.

基金项目

辽宁省教育厅科学研究基金项目(L2010265) (L2010265)

辽宁省农业科技攻关重点项目(2011214001) (2011214001)

辽宁省"百千万人才工程"培养经费资助项目(2010921043). (2010921043)

中国畜牧兽医

OA北大核心CSTPCD

1671-7236

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