中国畜牧兽医2012，Vol.39Issue(11)：215-219,5.
地衣芽孢杆菌M109高密度发酵条件的优化
Optimization of Culture Medium and Fermentation Conditions of Bacillus licheniformis M109
摘要
Abstract
Optimization of Bacillus licheniformis M109 culture medium in this research based on the orthogonal test, carbon source, nitrogen source and carbon nitrogen ratio were determine as necessary for fermentation medium of Bacillus licheniformis M109. In order to enhance Bacillus licheniformis M109 fermentation level, growth curve of Bacillus licheniformis M109 and trend of Ph, dissolved oxygen(DO) were studied. Though studying the fermentation process of growth, the optimal Ph and inoculation amount were determined. The results found that DO was a key factor that limited the growth of Bacillus licheniformis M109. It was studied regulation rotate speed of stirring to improve dissolved oxygen and the fermentation density in culture medium. Another study found that Bacillus licheniformis M109 fermentation level could be improved by adding fresh medium. Through optimization of culture medium and conditions, the fermentation level of Bacillus licheniformis M109 incneased to 1. 2×1010 CFU/Ml from the initial 1. 0×109 CFU/Ml and the rate of bacillus arrived to 88%.关键词
地衣芽孢杆菌/培养基/发酵条件/优化Key words
Bacillus licheniformis / culture medium/ fermentation condition/ optimization分类
生物科学引用本文复制引用
付维来,杜建涛,刘鹏,王安如..地衣芽孢杆菌M109高密度发酵条件的优化[J].中国畜牧兽医,2012,39(11):215-219,5.基金项目
"973"课题"有益微生物对动物的生物学活性剂功能研究"(2010CB35703) （2010CB35703）
海淀区重大项目"新型饲用乳酸菌产品的研制与产业化"(K20100130Z)资助. （K20100130Z）
