中国畜牧兽医2012，Vol.39Issue(12)：40-44,5.
致病性嗜水气单胞菌PCR快速检测方法的建立
Establishment of PCR Method for Rapid Detection of Pathogenic Aeromonas hydrophila
摘要
Abstract
PCR method to detect 16S rDNA gene and aerolysin gene of Aeromonas hydrophila was established, in which two pairs of primers according to the conserved 16S rDNA gene and aerolysin gene sequence of Aeromonas hydrophila were used. 12 strains were tested for pathogenic A. Hydrophila by PCR, the results showed that all the 12 strains of Aeromonas hydrophila contain 16S rDNA gene, and 3 strains contain Aerolysin gene. BLAST analysis showed that the amplified sequences were coincident with the published 16S rDNA gene and Aerolysin gene of Aeromonas hydrophila on GenBank.关键词
嗜水气单胞菌/16S rDNA/Aero基因Key words
Aeromonas hydrophila / 16S rDNA/ Aero gene分类
生物科学引用本文复制引用
朱芝秀,邓舜洲,杨竹青,邬向东,万根,张文波,罗红亮..致病性嗜水气单胞菌PCR快速检测方法的建立[J].中国畜牧兽医,2012,39(12):40-44,5.基金项目
江西省教育厅(GJJ12237)课题 （GJJ12237）
江西科技厅(20122BBF60082)课题. （20122BBF60082）
