中国畜牧兽医2012，Vol.39Issue(12)：128-132,5.
中国地鼠微卫星DNA引物的设计及筛选
Designing and Screening of Microsatellite Primers in Chinese Hamster
摘要
Abstract
The objectives of the present study were to screen new microsatellite loci in Chinese hamster to develop genetic markers for genetic analysis. A library of partial small size fractionated genomic DNA was constructed with the Chinese hamster. 135 pairs of primers were designed with the software Primer 3. 0 for rnicrosatellites positive clones obtained. 11 pairs primers that the stutter bands easily in theory were synthesized and 20 samples were tested with them. The results showed 10 of the 11 loci were found to be polymorphic,and PIC, the mean observed heterozygosities (Ho) , the mean expected heterozy-gosities( He) were 0. 4195, 0. 3895 and 0. 4565, respectively. The microsatellite markers would be useful for further studying on accessions identification and breeding of Chinese hamster,which would provide some evaluable tools for marker-assisted selection breeding and gene mapping in Chinese hamster.关键词
中国地鼠/微卫星/基因组文库Key words
Chinese hamster/microsatellite/enriched library分类
农业科技引用本文复制引用
宋国华,耿佳宁,贾若愚,岳文斌,刘田福,胡松年..中国地鼠微卫星DNA引物的设计及筛选[J].中国畜牧兽医,2012,39(12):128-132,5.基金项目
山西省实验动物专项资金资助(2005K01). （2005K01）
