中国畜牧兽医2012，Vol.39Issue(12)：159-161,3.
高效液相色谱法测定大鼠肝微粒体温孵体系中血根碱的含量
Determination of Sanguinarine in Rat Liver Microsomes Incubation System by HPLC
摘要
Abstract
A HPLC method for the determination of sanguinarine in rat liver microsomes incubation system was developed. Chromatography was performed on a Shimadzu VP-ODS column (150 mm×4. 6 mm,5 μm). An aeetonitrile-0. 1% phosphoric acid solution (V :V=30:70) as the mobile phase was used on the wavelength of 284 nm. The recovery of 500 and 1000 nmol/L control solution was 98. 3% and 98. 9% respectively. The relative standard deviation of 250, 500 and 1000 nmol/L standard solution for the within-day and between-day were 3. 76%, 2.88%, 3.56% and 5.19%, 4.96%, 3.84%, respectively. The calibration curve was linear in the range from 250 to 10000 nmol/L with r=0. 9997. The method was accurate, sensitive and rapid for the determination of sanguinarine in rat microsomes incubation system.关键词
血根碱/高效液相色谱法/大鼠/肝微粒体Key words
sanguinarine/ high performance liquid chromatography/ rat/ liver microsomes分类
农业科技引用本文复制引用
姚芳芳,朱若岑,刘兆颖,田世杰,唐昭山,刘琪,伍勇..高效液相色谱法测定大鼠肝微粒体温孵体系中血根碱的含量[J].中国畜牧兽医,2012,39(12):159-161,3.基金项目
湖南农业大学东方科技学院大学生科技创新项目(DFCXY201160). （DFCXY201160）
