中国畜牧兽医2012，Vol.39Issue(12)：212-215,4.
根黄分散片的含量测定及制剂稳定性研究
Determination of Genhuang Dispersible Tablets by RP-HPLC and the Study of the Stability
摘要
Abstract
The study aimed to establish a method for determining Baicalin in Genhuang dispersible tablets and inspection for the preparation of stability. RP-HPLC method was adopted and the C18 column(150 mm×4. 6 mm, 10μm)was used. The mobile phase was methanol-water-phosphoric acid(47:53: 0. 2%), with the flow rate of 1 mL/min. The detection wavelength was at 280 nm and the column temperature was 30 ℃. Under the high-light exposure experiment, accelerating experiment and room temperature storage observation, the results showed that the product was basically stable. The calibration curve of Baicalin was linear in the range of 1. 018 to 50. 90 μg/mL, r=0. 9999(n=6) , the average recovery rate was 97. 27% , RSD=0. 75% (n=9). The method was simple, sensitive and accurate with good repeatability, which can be used for the quality control of Genhuang dispersible tablets, and the preparation of good stability.关键词
反相高效液相色谱法/根黄分散片/黄芩苷/稳定性Key words
RP-HPLC/ Genhuang dispersible tablets/ Baicalin/ stability分类
农业科技引用本文复制引用
辛蕊华,罗永江,郑继方,李维,王贵波,罗超应,李锦宇,谢家声..根黄分散片的含量测定及制剂稳定性研究[J].中国畜牧兽医,2012,39(12):212-215,4.基金项目
"十二五"国家科技支撑计划项目资助(2011BAD34B03) （2011BAD34B03）
中央级公益性科研院所基本科研业务费专项资金项目(BRF090404). （BRF090404）
