中国循证儿科杂志2012，Vol.7Issue(6)：459-464,6.DOI:10.3969/j.issn.1673-5501.2012.06.012
谷氨酰胺对内毒素血症新生幼鼠肠道功能保护作用的研究
The effect of glutamine on gastrointestinal protection in lipopolysaccharide-induced newborn rats
摘要
Abstract
Objective To study the protective effects of glutamine( Gln ) on intestinal mucosa in lipopolysaccharide-induced newborn rats. Methods Four broods of mother and infant rats were randomized into four groups: methionine sulfoximine( MS ) + Gln group: Gln-deprived pups + Gln supplementation ;MS + NS group: Gln-deprived pups without Gln supplementation; Normally fed and Gln group: normally fed pups + Gln supplementation; Normally fed and NS group: normally fed pups without Gln supplementation; MS,NS and Gln were given by intraperitoneal injection for 6 days. All pups received lipopolysaccharide ( LPS ) on D7 and were sacrificed 6 hours later. Small intestine was harvested for studies. Plasma Gln concentration was detected by high efficiency liquid chromatography.IL-1, TNF-α and MCP-1 levels were determined with homogenate by ELISA. The intestines were examined under both light, transmission and scanning electron microscopy ( EM ) and membrane fluidity was determined with fluorescent polarization, using a DPH probe. Results ①The number of pups in 4 groups was 11, 11, 10 and 11 respectively, but when the experiment was finished, the number was 9, 5, 10 and 11 respectively. ② Pups weight gain ( g), plasma Gln concentration( μmol o L-1 ), IEC membrane fluorescence polarization and cytokine expression ( MCP-1, TNF-α, IL-1 β pg o mL-1 )of four groups were( 1.52 ±0.64),(0.58 ±0. 77),( 11.72 ±1.03)and( 11. 80 ± 1. 45 ),( 394. 3 ±92. 5 ),( 105.9 ± 37. 9),(774.6±143.7)and(676.8 ± 113.6 ),( 0.241 ±0. 046 ),( 0. 297 ±0.033 ),( 0.215 ±0. 052 )and ( 0. 211 ±0.049), (8.0±1.6)、(5.8 ±0. 8)、(8.5 ±1.3 )and( 8. 7 ± 1. 2 ),( 2. 2 ±0. 8 ),( 1. 3 ±0. 4 ),( 2. 8 ±1.0)and(2.5 ±1.1 ),( 28.5 ± 15. 5 ),( 16. 8 ± 11. 0 ),( 31. 4 ± 15. 1 )and( 32. 3 ± 13. 9 ). Overall, the between-group differences were statistically significant ( P ＜0. 05 ). ③Under light microscopy, the intestinal structure of MS + NS group pups was not intact, small intestine villi were the shortest and badly destroyed under optical microscopy. In MS + Gln group, the intestinal structure was also not intact, small intestine villi were shortened and destroyed, but some of them were restored. In normal + Gln and normal + NS group, small intestine villi were slightly destroyed. ④Under transmission EM and scanning EM, the small intestine microvilli of MS + NS group pups were shortest, badly abrupt and disorderly arranged, the membranes were destroyed and mitochondria were obviously ruptured. In MS + Gln group, small intestine microvilli were shorter and disorderly arranged, the membranes were destroyed and mitochondria were obviously swollen. In normal + Gln and normal + NS group, small intestine microvilli were normally arranged and not obviously ruptured, the membranes were not destroyed. Conclusions Gln deprivation adversely affected gastrointestinal structure and function of newborn rats. Glutamine supplementation was protective against the damage of the gastrointestinal tract caused by endotoxemia.关键词
谷氨酰胺/蛋氨酸亚砜胺/脂多糖/细胞膜脂质流动性Key words
Glutamine/ Methionine sulfoximine/ Lipopolysaccharide/ Membrane fluidity引用本文复制引用
苗士建,张烨,黄瑛,陈莲..谷氨酰胺对内毒素血症新生幼鼠肠道功能保护作用的研究[J].中国循证儿科杂志,2012,7(6):459-464,6.基金项目
上海市自然科学基金:062R14017 （）
卫生部国家临床重点专科建设项目 （）
