中国药房2012，Vol.23Issue(39)：3667-3669,3.DOI:10.6039/j.issn.1001-0408.2012.39.07
因宁片对甲亢性肝损害模型大鼠肝脏细胞色素P450基因表达的影响
Effects of Yinning Tablet on Gene Expression of Cytochrome P450 in Liver Tissue of Rats with Hyperthyroidism Liver Damage
周静 1王成蹊 1潘弟仪 1周涛 1侯连兵1
作者信息
- 1. 南方医科大学南方医院药学部,广州510515
- 折叠
摘要
Abstract
OBJECTIVE: To study the effects of Yinning tablet on gene expression of cytochrome P450(CYP450) in liver tissue of rats with hyperthyroidism liver damage. METHODS: Rats were perfused with levothyroxine sodium intragastrically to establish the rat model of hyperthyroidism liver damage. The model rats were divided into 6 groups, I.e. Blank control group, model group, Ji-akangning group and Yinning tablet high-dose, medium-dose and low-dose groups (2.4, 1.2,0.6 g·kg-1). The expressions of CYP1A2,CYP2E1 and CYP3A2 in liver tissue were detected by real-time quantitative PCR. RESULTS: Compared with model group, the expressions of CYP2E1 was decreased significantly(P<0.05), and the expressions of CYP1A2 and CYP3A2 had no significant change(P>0.05). CONCLUSION: Yinning tablet may down-regulate the expression of CYP2E1 in liver tissue of rats with hyperthyroidism liver damage, which may be one of the molecular action mechanisms that Yinning tablet prevent hyperthyroidism liver damage.关键词
因宁片/甲状腺功能亢进/肝损害/大鼠/细胞色素P450/实时荧光定量聚合酶联反应Key words
Yinning tablet/ Hyperthyroidism/ Liver damage/ Rat/ CYP450/ Real-time quantitative PCR分类
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周静,王成蹊,潘弟仪,周涛,侯连兵..因宁片对甲亢性肝损害模型大鼠肝脏细胞色素P450基因表达的影响[J].中国药房,2012,23(39):3667-3669,3.
