中国药房2012，Vol.23Issue(39)：3699-3700,2.DOI:10.6039/j.issn.1001-0408.2012.39.18
HPLC-ELSD法测定山茱萸萜中齐墩果酸与熊果酸的含量
Content Determination of Oleanolic Acid and Ursolic Acid in Terpene of Cornus officinalis by HPLC-ELSD
摘要
Abstract
OBJECTIVE: To establish a method for the content determination of oleanolic acid and ursolic acid in terpene of Cornus officinalis. METHODS: HPLC-ELSD method was adopted. The determination was performed on welchrom-C18 column (250 mm×4.6 mm, 5 μm) with mobile phase consisted of methanol-1% ammonium acetate (85:15, V/V) at the flow rate of 0.8 mL-miiT' and column temperature of 30 ℃. The temperature of drift tube was 90 ℃, and flow rate was 2.4 L-min-1. RESULTS: The linear range was 15.00～75.00 μg·mL-1 for oleanolic acid and 60.00～300.00 μg·mL-1 for ursolic acid (r=0.999 9); their average recovery rates were 99.14%(RSD= 1.46% ,n=6) and 97.37% (RSD= 1.79%, n=6). CONCLUSION: The method is accurate and reliable. It can be used for quality control of terpene of C. Officinalis.关键词
山茱萸萜/高效液相色谱-蒸发光散射检测法/齐墩果酸/熊果酸/含量测定Key words
Terpenes of Cornus officinalis/ HPLC-ELSD/ Oleanolic acid/ Ursolic acid/ Content determination分类
医药卫生引用本文复制引用
黄和军,许惠琴,崔小兵..HPLC-ELSD法测定山茱萸萜中齐墩果酸与熊果酸的含量[J].中国药房,2012,23(39):3699-3700,2.基金项目
国家自然科学基金资助项目(81073111) （81073111）
