中国药房2012，Vol.23Issue(40)：3823-3825,3.DOI:10.6039/j.issn.1001-0408.2012.40.29
紫外-可见分光光度法测定鱼腥草注射液中聚山梨酯80的含量
Content Determination of Polysorbate 80 in Yuxingcao Injection by UV-visible Spectrophotometry
吴春丽 1李爱星 1李杰明 1魏会杰 1马慧艳 1乔家彬1
作者信息
摘要
Abstract
OBJECTIVE: To establish the method for content determination of polysorbate 80 in Yuxingcao injection. METHODS : The content of Yuxingcao injection was determinated by UV-visible spectrophotometry at the detection wavelength of 623.50 nm using water as blank control based on the blue complex that was the reaction product of polyethoxy of polysorbate 80 and ammonium cobalt thiocyanate, which can dissolved in dichloromethane. RESULTS: The linear range of polysorbate 80 was 0.053— 0.530 mg·mL-1 (r=0.999 9) with an average recovery of 100.38% (RSD= 1.04% , n=9). CONCLUSION: The method is simple, easy, accurate, reliable, stability and exclusive, and it is suitable for the quality control of Yuxingcao injection.关键词
鱼腥草注射液/聚山梨酯80/紫外-可见分光光度法/含量测定Key words
Yuxingcao injection/ Polysorbate 80/ UV-visible spectrophotometry/ Content determination分类
医药卫生引用本文复制引用
吴春丽,李爱星,李杰明,魏会杰,马慧艳,乔家彬..紫外-可见分光光度法测定鱼腥草注射液中聚山梨酯80的含量[J].中国药房,2012,23(40):3823-3825,3.
