中国药房2012，Vol.23Issue(41)：3882-3884,3.DOI:10.6039/j.issn.1001-0408.2012.41.16
甘脯酰阿霉素对斑马鱼胚胎的毒性评估
Evaluation of the Toxicity of Z-GP-Dox to Zebrafish Embryos
赵海山 1赵成建 2张欢 1蔡绍晖1
作者信息
- 1. 暨南大学药学院,广州510632
- 2. 四川大学生物治疗国家重点实验室,成都610041
- 折叠
摘要
Abstract
OBJECTIVE: To investigate the acute toxicity, cardiotoxicity and peripheral blood toxicity of N-carbobenzoxy-gly-cyl-proline-Dox (Z-GP-Dox) to zebraflsh embryos. METHODS: With 24 hpf (duration after fertilization) zebrafish embryos as model and DMSO as solvent, the effets of 1.25, 2.5, 5, 10, 15 and 20 μmol·L-1 Z-GP-Dox and Dox on survival rate of 48, 72 and 96 hpf embryos were investiagted to study acute toxicity; the effects of 5 and 10 u.mol-L～' Z-GP-Dox and Dox on survival rate of 72 hpf embryos were investiagted to study cardiotoxicity; the effects of Z-GP-Dox and Dox on the distribution and the number of red blood cell and neutrophil cell were investiagted to study peripheral blood toxicity, compared with solvent control group. RESULTS: Solvent had no significant effect on embryos survival. Less than 25% embryos were alive after 96 hpf when Dox>10 μ mol·L-1, while more than 70% embryos were suvival at 20 μmol·L-1 Z-GP-Dox. Compared with solvent control group, 5 and 10 μ mol·L-1 Dox could reduce heart rate and the number of neutrophil cell; 5 and 10 μmol·L-1 Z-GP-Dox had no significant effect on reducing heart rate and the number of red blood cell and neutrophil cell. CONCLUSION: The toxicity of Z-GP-Dox is significantly lower than that of Dox.关键词
甘脯酰阿霉素/斑马鱼胚胎/急性毒性/心脏毒性/外周血液毒性Key words
Z-GP-Dox/ Zebrafish embryos/ Acute toxicity/ Cardiotoxicity/ Peripheral blood toxicity分类
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赵海山,赵成建,张欢,蔡绍晖..甘脯酰阿霉素对斑马鱼胚胎的毒性评估[J].中国药房,2012,23(41):3882-3884,3.
