中国药房2012，Vol.23Issue(41)：3906-3907,2.DOI:10.6039/j.issn.1001-0408.2012.41.26
旋光法测定硫酸西索米星氯化钠注射液中西索米星的含量
Content Determination of Sisomicin in Sisomicin Sulfate and Sodium Chloride Injection by Polarimetry
戴月华 1邰顺章 2王冲3
作者信息
- 1. 盐城市第三人民医院,江苏盐城224001
- 2. 盐城市药品检验所,江苏盐城224002
- 3. 南京中医药大学,南京210046
- 折叠
摘要
Abstract
OBJECTIVE: To establish the method for content determination of sisomicin in Sisomicin sulfate and sodium chloride injection (SSJ). METHODS: The optical rotation-standard curve method (polarimetry) was adopted for the content determination of sisomicin in SSJ. The D line of sodium spectrum (589.3 nm) was adopted to determine the optical rotations with the length
of determination tube of 2 dm and determination temperature of 20 ℃, and it was compared with standard method--microbial
method. RESULTS: The linear range of sisomicin was 0.4～2.0 mg·mL-1 (r=0.999 9) with an average recovery of 97.6% (RSD= 0.80%, n=6); results of polarimetry (RSD=0～0.07%) was in line with that of microbial method (RSD=0.57%～1.2%). CONCLUSION : The method is simple and accurate, and the reproducibility of it is better than microbial method.关键词
硫酸西索米星氯化钠注射液/旋光法/微生物检定法/含量测定Key words
Sisomicin sulfate and sodium chloride injection/ Polarimetry/ Microbial method/ Content determination分类
医药卫生引用本文复制引用
戴月华,邰顺章,王冲..旋光法测定硫酸西索米星氯化钠注射液中西索米星的含量[J].中国药房,2012,23(41):3906-3907,2.
