中国药房2012，Vol.23Issue(42)：3968-3969,2.DOI:10.6039/j.issn.1001-0408.2012.42.13
HPLC法测定人血浆中5-氟尿嘧啶的浓度
Determination of 5-fluorouracil Concentration in Human Plasma by HPLC
叶丽卡 1谢志红 1陈瑞卿1
作者信息
- 1. 广州医学院第二附属医院,广州510260
- 折叠
摘要
Abstract
OBJECTIVE: To develop a method for the determination of 5-fluorouracil (5-FU) concentration in human plasma. METHODS: Plasma sample was treated with twice extraction of ethyl acetate, and then HPLC method was adopted for concentration determination. The determination was performed on Ultimate C18 column with mobile phase consisted of 10 mmol·L-1 KH2PO4 at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 265 nm and column temperature was 40 ℃. RESULTS: The linear range of 5-FU was 0.48～61.4 μg·mL-1(n=0.999 8). The lowest limit of determination was 0.48 μg·mL-1. The RSD of in
tra-day and inter-day were 5.12%～9.98% and 5.28%～10.95% respectively (n=5). The recovery rates were all higher than 85% at high, medium and low conentrations. The samples were stable in room and low temperature, and were also stable in frozen and thawed condition. CONCLUSION: This method is simple, precise and stable, and it is suitable for the determination of 5-FU concentration in human plasma.关键词
5-氟尿嘧啶/血药浓度/高效液相色谱法Key words
5-fluorouracil/ Plasma concentration/ HPLC分类
医药卫生引用本文复制引用
叶丽卡,谢志红,陈瑞卿..HPLC法测定人血浆中5-氟尿嘧啶的浓度[J].中国药房,2012,23(42):3968-3969,2.
