中国药房2012，Vol.23Issue(43)：4082-4083,2.DOI:10.6039/j.issn.1001-0408.2012.43.19
RP-HPLC法测定不同产地不同部位贯叶金丝桃中金丝桃苷的含量
Content Determination of Hyperoside in Different Parts of Hypericum perforatum from Different Habitats by RP-HPLC
摘要
Abstract
OBJECTIVE: To develop the method for the content determination of hyperoside in Hypericum perforatum, and to determine the content of hyperoside in different parts of H. perforatum from different habitats. METHODS: RP-HPLC method was adopted. The determination was performed on Boston Breeze-C18(250 mm×4.6 mm, 5 μm) column with mobile phase consisted of methanol-0.1% phosophoric acid(40:60, VIV) at the flow rate of 1.0 mL-min-1. The detection wavelength was set at 360 nm. RESULTS: The linear rang of hypericin was 0.14~2.80μg-mL-1(r= 0.999 4) with an average recovery of 95.97% (RSD= 1.16% , n=6). The contents of hypericin in the flowers and leaves of H, perforatum from Sichuan province were the highest. CONCLUSION: The method is simple, accurate and reproducible for the quality control of H. perforatum.关键词
贯叶金丝桃/金丝桃苷/反相高效液相色谱法/含量测定/产地Key words
Hypericum perforatum/ Hyperoside/ RP-HPLC/ Content determination/ Habitats分类
医药卫生引用本文复制引用
李卓恒,孟德胜,卢来春,周薇,于彩平,藕顺龙,吴畏..RP-HPLC法测定不同产地不同部位贯叶金丝桃中金丝桃苷的含量[J].中国药房,2012,23(43):4082-4083,2.基金项目
国家自然科学基金资助项目(81073157) （81073157）
重庆市科技攻关课题(CSTC,2010AC5023) （CSTC,2010AC5023）
