中国医科大学学报2012，Vol.41Issue(12)：1077-1079,3.
Shaker通道电压感受器S3-S4片段末端氨基酸分析
Mapping the Ends of S3-S4 Segments of Voltage Sensor in Shaker Channel
摘要
Abstract
Objective To analyze the membrane positions of S3-S4 segments in voltage sensor of traditional Shaker channel. Methods Membrane topological analysis and glycosylation scanning methods were used. Results In Shaker channel, S3 inserts into membrane with an Nexc/Ccyt orientation and the C-terminus ends at T329 residue ;S4 inserts into membrane with an Nexc/Ccyt orientation and the N-terminus ends at L358 residue. Conclusion S3 and S4 of Shaker channel are transmembrane segments; our findings are consistent with the traditional Shaker model which supports voltage gating mechanism.关键词
Shaker通道/电压门控机制/膜拓扑结构/电压门控钾通道Key words
Shaker channel/voltage gating mechanism/membrane topology/Kv channel分类
生物科学引用本文复制引用
尚德志,张丽艳,汲坤,沈薇,牟均..Shaker通道电压感受器S3-S4片段末端氨基酸分析[J].中国医科大学学报,2012,41(12):1077-1079,3.基金项目
国家自然科学基金资助项目(30971177) （30971177）
辽宁省教育厅重点实验室项目(LS2010162) （LS2010162）
