中国医科大学学报2012，Vol.41Issue(12)：1095-1098,4.
不同糖浓度中大鼠DRG神经元相关蛋白表达及Ca2+浓度的测定
The Expression of Related Proteins and Calcium Level in DRG Neuron within Different Glucose Concentration
摘要
Abstract
Objective To observe the effect of high glucose concentration on transient receptor potential vanilloid 4 (TRPV4),protein ki-nase C (PKCε )and intercellular Ca2+ concentration in DRG neurons of the rats and reveal the mechanism of painful diabetic neuropathy. Methods The DRG neurons were isolated from neonate rat. The neurons were divided into control(C),medium(M ) and high glucose(H) group which were primarily cultured in medium with different glucose concentration. After 48 h, the expression of TRPV4 and PKCε was de-tected by Western blotting. The Ca2+ concentration was detected by laser scanning confocal microscope. Results TRPV4 and PKCε in-creased in dose dependent manner in different glucose concentration. TRPV4 were 3.20±0.40 and 7.69±0.60 in M and H group,but C group was 0.33±0.05. PKCε showed 1.08±0.08 and 1.97±0.35 in M and H group,but C group was 0.88±0.04. 4α-PDD,a TRPV4 activator can raise intracellular calcium in dose-dependent manner,H group was significant (P< 0.05 vs control). Conclusion High glucose concentra-tion can active the expressions of TRPV4,PKCε in DRG neurons in rats. The responsibility of Ca2+ concentration of DRG neuron is accentu-ated in high glucose concentration. TRPV4 and PKCε both donate the augmented Ca2+ concentration in DRG neurons. High glucose levels in-crease the reactivity of the DRG neuron.关键词
痛性糖尿病周围神经病变/瞬时感受器电位香草酸受体4/蛋白激酶Cε/钙离子Key words
painful diabetic neuropathy/transient receptor potential vanilloid 4/protein kinase Cε/Ca2+分类
医药卫生引用本文复制引用
丁炎,那存乌力吉,阎雪晶,姚维凡,赵海山,陈磊..不同糖浓度中大鼠DRG神经元相关蛋白表达及Ca2+浓度的测定[J].中国医科大学学报,2012,41(12):1095-1098,4.基金项目
辽宁省教育厅高校科研计划(2009A763) （2009A763）
