中国医科大学学报2012，Vol.41Issue(12)：1099-1101,3.
103钯放射性支架诱导胆管癌细胞凋亡及对抑癌基因的影响和意义
Significance of 103pd Radioactive Stent Induced Cholangiocarcinoma Cells Apoptosis and Effects on Tumor Suppressor Genes
摘要
Abstract
Objective To investigate the role of tumor suppressor genes {p53 gene) in radioactive induced eholangiocareinoma cells apoptosis. Methods A total of 12 nude mire were randomly divided into 103pd radioactive stent group (n =6) and control group (n -6). Cross-sections were stained by HE for cholangiocarcinoma. The expression of p53 in cholangiocareinoma tissue was detected using in situ hy-bridization, cells with the brow-yellow nucleus after DAB coloration indicates positive staining. Positive rate was analyzed under microscope and apoptosis cell in paraffin-bedding cholangiocarcinoma tissue section was determined using immunohistologic-al staining method. Results Expression of p53 was higher in the 103pd radioactive stent group than that in the control group (P < 0.01). The cholangiocarcinoma cell apoptosis rate was significantly increased in the 103pd radioactive stent group than the control group (P < 0.01). Conclusion These results suggest that p53 gene was increased by 103pd induced chokngiaaremoma cell apoptosis, the, results also show that 103pd radioactive stent can inhibit cholangiocarcinoma cell proliferation.关键词
p53基因/细胞凋亡/γ射线Key words
p53 gene/apoptosis/γ-ray分类
医药卫生引用本文复制引用
何贵金,姜维国,郭启勇,纪大伟,顾玺,王柳,赵晓丹,潘春来,李安,许书河,高沁怡..103钯放射性支架诱导胆管癌细胞凋亡及对抑癌基因的影响和意义[J].中国医科大学学报,2012,41(12):1099-1101,3.基金项目
辽宁省自然科学基金资助项目(20042063) （20042063）
辽宁省教育厅高校科研计划(05L445) （05L445）
辽宁省科技计划项目(2006225001-6) （2006225001-6）
沈阳市科技计划项目(F12-193-9-01) （F12-193-9-01）
