中国药理学通报2012，Vol.28Issue(12)：1686-1689,4.DOI:10.3969/j.issn.1001-1978.2012.12.013
miR-96在人乳腺癌中表达及其对乳腺癌细胞侵袭和迁移活性的影响
Expression of miR-96 in breast cancer and its role in invasion and migration of breast cancer cells
摘要
Abstract
Aim To investigate the expression of miR-96 in breast cell lines and breast tumor tissue specimens and the impact on the biological behaviors of breast cancer cells. Methods Total miRNAs were isolated from 4 breast cell lines and 28 tumor tissue specimens of breast disease. The expression of miR-96 was determined by real-time PCR assay on those breast cell lines and tissue specimens. The characteristics of cell proliferation, invasion and migration were examined after miR-96 inhibitor transfection by lipofection. Results Altered expression of miR-96 was observed in the highly invasive breast cancer cells, compared with weakly invasive breast cells. The expression of miR-96 was significantly decreased in the breast cancer tissues, compared with benign breast diseased tissues. The capacity of breast cancer cell invasion and migration was significantly increased after transfection of miR-96 inhibitor. No significant change was observed on the cell proliferation by miR-96 inhibitor transfection. Conclusion Altered expression of miR-96 maybe plays an important role in breast cancer development and progression.关键词
乳腺癌/miRNA/miR-96/增殖/侵袭/迁移Key words
breast neoplasms/ miRNA/ miR-96/ proliferation invasion/ migration分类
医药卫生引用本文复制引用
吴正升,吴强..miR-96在人乳腺癌中表达及其对乳腺癌细胞侵袭和迁移活性的影响[J].中国药理学通报,2012,28(12):1686-1689,4.基金项目
国家自然科学基金资助项目(No 81172533,30873047,81101597) （No 81172533,30873047,81101597）
