中国药理学通报2012，Vol.28Issue(12)：1714-1718,5.DOI:10.3969/j.issn.1001-1978.2012.12.019
二烯丙基二硫下调LIMK1抑制人胃癌MGC803细胞迁移与侵袭
Downregulation of LIMK1 expression inhibits migration and invasion of human gastric cancer MGC803 cells by diallyl disulfide
摘要
Abstract
Aim To investigate the effects of DADS on the migration and invasion of human gastric cancer MGC803 cells and the effects on LIMK1 expression. Methods Abilities of proliferation, migration and invasion in MGC803 cells by DADS were examined by MTT test, Wound healing assay and transwell membrane assays. The effects of LIMK1 expression in MGC803 ceUs by DADS were detected by RT-PCR and Western blot. Results MTT test showed that the inhibitory rates were 31.8%, 66.1%, 83.6% and 89. 2% , respectively for 24 h, 48 h, 72 h and 96 h in MGC803 cells exposed to 30 mg · L-1 DADS, which exhibited a time-dependent model ( P <0. 05 ). Wound healing assays displayed that the scratch healing distances were significantly broadening in a dose-dependent manner, compared with the control group for 24 h in MGC803 cells treated with 10, 20, 30, 40, 50 mg · L-1 DADS ( P < 0. 05 ) respectively. Transwell experiments exhibited that the numbers of treated group cells through matrigel were 35. 8 ± 3. 74, 34. 1 ± 2. 02, 31. 7 ± 4. 81, 17. 2 ± 3. 08 and 13. 2 ± 3. 36, respectively, significantly lower than those in the control group ( 39. 5 ± 2. 99 ) in a dose-dependent manner ( P <0. 05 ). Compared with the negative control group, the expression of LIMK1 mRNA was significantly inhibited in MGC803 cells after treated with 30 mg · L-1 DADS examined by RT-PCR ( P < 0. 05 ). Moreover, Western blot showed that DADS reduced expressions of LIMK1 protein in a time-dependent manner ( P < 0. 05 ). Conclusions DADS can significantly inhibit the capability of migration and invasion of MGC803 cells. The potential molecular mechanisms may be related to the downregulation of LIMK1 expression.关键词
胃癌/MGC803细胞/二烯丙基二硫/迁移/侵袭/LIMK1Key words
gastric cancer/ MGC803 cells/ Diallyl disulfide/ migration/ invasion/ LIMK1分类
医药卫生引用本文复制引用
马艳华,苏波,向姝霖,姜浩,杨邦敏,章硕,夏红,苏琦..二烯丙基二硫下调LIMK1抑制人胃癌MGC803细胞迁移与侵袭[J].中国药理学通报,2012,28(12):1714-1718,5.基金项目
国家自然科学基金资助项目(No 81102854) （No 81102854）
湖南省教育厅科学研究重点项目(No 09A077) （No 09A077）
