中国药理学通报2012，Vol.28Issue(12)：1719-1723,5.DOI:10.3969/j.issn.1001-1978.2012.12.020
双氢青蒿素对人骨肉瘤细胞株143B增殖和凋亡的作用
Role of dihydroartemisinin(DHA) in proliferation and apoptosis of human osteosarcoma 143B cells
摘要
Abstract
Aim To observe the effect of dihydroarte-misinin ( DHA ) on proliferation and apoptosis of human osteosarcoma 143B cells and the possible mechanism. Methods Human osteosarcoma cell line 143B was cultured in vitro; MTT [3 -(4,5) the-dimethylthi-ahiazo ( -z-y1)-3 ,5-di-phenytetrazoliumromide,MTT ] colorimetric assay and clone formation assay were applied to detect the role of DHA in the survival and growth status of human osteosarcoma cells. Hoechst 33258 cell staining was used for apoptosis detection. The luciferase reporter gene, β-Catenin-Luc ( p-Top ) was constructed to detect β-Catenin activity after DHA' s role in 143B cells. The marker protein expression closely related to tumor cell proliferation, such as PCNA,Cyclin D1 ,C-Myc,and apoptosis,such as Bad, Bcl-2, Caspase-3, was detected by Western blot. Results 24 hours after DHA played role in 142B, MTT results showed that DHA could inhibit the proliferation of the 143B cells, and colony formation diminished( P < 0. 05 ), the inhibition efficiency increased significantly at a concentration of 35 μmol · L-1DHA. 143B cells was significantly enhanced ( P < 0. 05 ), and the PCNA expression was lowered, and cell proliferation was inhibited, pro-apoptotic protein Bad and caspase-3 expression was significantly enhanced, while Bcl-2 protein expression decreased significantly. Conclusions Dihydroartemisinin significantly inhibits the proliferation of human osteosarcoma 143B cells and promote apoptosis, which may be associated with the downregula-tion of cell proliferation-associated proteins, the PCNA and Bcl-2, and the upregulation of pro-apoptotic protein, Bad and Caspase-3.关键词
双氢青蒿素/人骨肉瘤细胞/细胞增殖/细胞凋亡/Wnt信号通路/β-CateninKey words
dihydroarteminin( DHA ) / human-osteosarcoma/ proliferation/ apoptosis/ Wnt signaling pathway / β-Catenin分类
医药卫生引用本文复制引用
刘跃亮,罗进勇,张彦,何通川,曾照芳..双氢青蒿素对人骨肉瘤细胞株143B增殖和凋亡的作用[J].中国药理学通报,2012,28(12):1719-1723,5.基金项目
国家自然科学基金资助项目(No 31071304,81172017 ) （No 31071304,81172017 ）
