中国药理学通报2012，Vol.28Issue(12)：1729-1732,4.DOI:10.3969/j.issn.1001-1978.2012.12.022
白花丹醌对人肝癌细胞HepG2、SMMC-7721增殖及其Bax/Bcl-2、Cyclin D1mRNA表达的影响
yuanliu163@yahoo.com.cn The influence of plumbagin on proliferation of the human hepatoma carcinoma cells HepG2, SMMC-7721 and mRNA expression of Bax/Bcl-2, Cyclin D1
摘要
Abstract
Aim To investigate the influence of plumbagin on the proliferation of human hepatoma carcinoma cells HepG2 and SMMC-7721 and further research the mechanism of the influence. Methods HepG2 and SMMC-7721 were respectively co-cultured with plumbagin. Then, through microscopic imaging and MTT assay, the effect of plumbagin on the two kinds of cells was studied; finally, with real-time fluorescence quantitative PCR ( Qpcr ) analysis, the mechanism behind the effect was investigated. Results Both microscopic imaging and MTT assay analyses showed that plumbagin could significantly inhibit the proliferation of the two kinds of cells, the further Qpcr analysis showed that for the two kinds of cells, plumbagin could significantly up-regulate the Bax/Bcl-2 ratio ( P = 0. 0017 and P = 0. 0104 ) , while significantly down-regulate the transcriptional level of Cyclin Dl ( P = 0. 0287 and P = 0. 0165 ). Conclusion Plumbagin can significantly inhibit the proliferation of HepG2 and SMMC-7721 , and the inhibitory mechanism is related to the increase of Bax/Bcl-2 ratio and the decrease of the transcriptional level of CyclinDl.关键词
白花丹醌/HepG2/SMMC-7721/Bax/Bcl-2/Cyclin D1Key words
cells HepG2 / human hepatoma carcinoma cells SMMC-7721/ Bax/ Bcl-2/ Cyclin Dl分类
医药卫生引用本文复制引用
张吉仲,万谦,刘圆..白花丹醌对人肝癌细胞HepG2、SMMC-7721增殖及其Bax/Bcl-2、Cyclin D1mRNA表达的影响[J].中国药理学通报,2012,28(12):1729-1732,4.基金项目
西南民族大学2012年度中央高校基本科研业务费专项(No 12NZYTH02) （No 12NZYTH02）
2012年度科技部支撑计划"藏、羌、彝等特色疗法规范及药物疗效评价技术研究"(No 2012BAI27B07) （No 2012BAI27B07）
四川省杰出青年学术技术带头人后续计划(No 2011JQ0051) （No 2011JQ0051）
