中国药业2012，Vol.21Issue(19)：9-11,3.
管碟法比较盐酸小檗碱和黄连药材对金色葡萄球菌的抑菌效力
Comparison of Antimicrobial Potency between Berberine Hydrochloride and Drug Material Coptis Chinensis on Staphyloccocus Aureus by Cup-Plate Method
谢梅 1徐春红 1胡正波 1卢海波 1余敏灵2
作者信息
- 1. 中国人民解放军第452医院,四川成都 610061
- 2. 四川省乐山市食品药品检验所,四川乐山 614000
- 折叠
摘要
Abstract
Objective To establish the cup - plate method for comparing the antimicrobial potency of berberine hydrochloride and Coptis chinensis on Staphyloccocus aureus. Methods With berberine hydrochloride as the reference substance and the inhibition zone diameter (D) as the response value, the experiment was designed according to the antibiotic bioassay cup - plate method in the appendix XIA of the Chinese Pharmacopoeia(edition 2010, Part 2). The antimicrobial potency of berberine hydrochloride and the sample of medicinal material Coptis chinensis was detected and compared. Results In the mass concentration ranges of 4.00-12.5 μg/mL for berberine hydrochloride and 66. 0 - 200 μg/mL for Coptis chinensis sample, the dose logarithm value respectively demonstrated better linear relation with the inhibition zone diameter ( r > 0. 99). The variation analysis between doses in the 3 - dose method and the reliability test results showed that regression was very significant ( P < 0. 01) and the deviation of parallelism, second degree curve, anti - second degree curve were not significant ( P > 0. 05). The experimental results were established. The difference within groups was very significant ( P < 0. 01). The average confidence limit was 1. 884 5％. Conclusion This biological assay method could be used for testing the antimicrobial potency of berberine hydrochloride and Coptis Chinensis.关键词
黄连/抑菌效力/管碟法/生物检定法/盐酸小檗碱Key words
Coptis chinensis/ antimicrobial potency/ cup - plate method/ biological assay/ berberine hydrochloride分类
医药卫生引用本文复制引用
谢梅,徐春红,胡正波,卢海波,余敏灵..管碟法比较盐酸小檗碱和黄连药材对金色葡萄球菌的抑菌效力[J].中国药业,2012,21(19):9-11,3.
