中国药业2012，Vol.21Issue(19)：11-12,2.
高效液相色谱-质谱联用法测定血浆中阿奇霉素浓度
Determination of Azithromycin in Human Plasma by HPLC-MS/MS
谭亚亚 1李博 2杨欣 3李巍4
作者信息
- 1. 西安正大制药有限公司,陕西西安 710043
- 2. 中国药科大学食品质量与安全教研室,江苏南京 210009
- 3. 教育部药物质量与安全预警重点实验室,江苏南京 210009
- 4. 陕西省食品药品检验所,陕西西安 710061
- 折叠
摘要
Abstract
Objective To develop an HPLC-MS/MS method for the determination of azithromycin in human plasma in order to evaluate its bioequivalence. Methods After adding internal standard, methanol was adopted for precipitating protein. After centrifugation, HPLC-MS/MS was performed for analysis on the C18 column with the mobile phase of 10 mmol/L ammonium acetate buffer solution (containing 0.1％ acetate acid) -methanol (35 : 65) and the selected fragment ions for detection. The healthy volunteers orally took the reference preparation and test preparation in a dose of 500 mg, respectively. After administration, the plasma azithromycin concentration was determined and the bioequivalence was calculated. Results The plasma azithromycin concentration in the range of 5. 91 -1 182 ng/mL demonstrated good linear relation. The lowest detection limit was 2. 37 ng/mL. Conclusion The established analytical method is sensitive, accurate, simple, convenient and suitable for the determination of plasma azithromycin.关键词
阿奇霉素/高效液相色谱-质谱联用/血药浓度Key words
azithromycin/ HPLC - MS/MS/ blood drug concentration分类
医药卫生引用本文复制引用
谭亚亚,李博,杨欣,李巍..高效液相色谱-质谱联用法测定血浆中阿奇霉素浓度[J].中国药业,2012,21(19):11-12,2.
