中国药业2012，Vol.21Issue(19)：41-42,2.
反相高效液相色谱法测定对氨基水杨酸钠片的有关物质
RP-HPLC Determination of Related Substances of Sodium Aminosalicylate Tablets
邓梅 1黄洁 1单敏1
作者信息
- 1. 陕西省西安市食品药品检验所,陕西西安 710054
- 折叠
摘要
Abstract
Objective To establish an HPLC method for the determination of related substances of Sodium Aminosalicylate Tablets. Methods The Phenomenex Luna C18 column(150 mm ×4. 6 mm, 5 μm) was adopted with methanol - 10％ tetrabutyl ammonium hydroxide solution - 0. 05 mol/L disodium hydrogen phosphate - 0. 05 mol/L sodium dihydrogen phosphate (200 : 19:400:400) as the mobile phase at the flow rate of 1.0 mL/min, the detection was set at 280 nm. Results Good separation of sodium aminosalicylate and aminophenol was achieved. The mass concentration of aminophenol in 0. 365 - 1. 825 μg/mL showed good linear ralation with the peak area. The detection limit was 0.012 μg/mL. Conclusion This method is simple, accurate and highly sensitive with good specificity selective, which can be used the detection method of related substances of Sodium Aminosalicylate Tablets.关键词
高效液相色谱法/对氨基水杨酸钠片/有关物质/间氨基酚Key words
HPLC/ Sodium Aminosalicylate Tablets/ related substance/ aminophenol分类
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邓梅,黄洁,单敏..反相高效液相色谱法测定对氨基水杨酸钠片的有关物质[J].中国药业,2012,21(19):41-42,2.
