中国药业2012，Vol.21Issue(21)：19-20,2.
反相高效液相色谱法测定长春西汀注射液的含量
Determination of Vinpocetine in Vinpocetine Injection by RP-HPLC
吴朝晖 1李根 2周静3
作者信息
- 1. 福建医科大学附属第一医院,福建 福州 350004
- 2. 四川省成都市妇幼保健院,四川 成都 610041
- 3. 四川大学华西药学院,四川 成都 610041
- 折叠
摘要
Abstract
Objective To establish an RP - HFLC method for determining the content of vinpocetine in Vinpocetine Injection. Methods RP - HPLC was performed on the Agilent C18 column(250 mm ×4. 6 mm, 5 μm). The mobile phase was methanol - 13 mmol/L ammonium carbonate solution - tetrahydrofuran(100 : 16:5). The flow rate was 1. 0 mL/min. The detective wavelength was set at 274 nm. Results The mass concentrations of vinpocetine within the range of 0. 2-1.0 g/L showed good linearity with the integral value of the peak area(r = 0.999 8). The average recovery rate was 99.35%, RSD was 0. 76% ( n = 9). Conclusion This method is simple, accurate and well reproducible, and can be used for the quality control of Vinpocetine Injection.关键词
长春西汀注射液/长春西汀/反相高效液相色谱法/含量测定Key words
Vinpocetine Injection/ vinpocetine/ RP - HPLC/ content determination分类
医药卫生引用本文复制引用
吴朝晖,李根,周静..反相高效液相色谱法测定长春西汀注射液的含量[J].中国药业,2012,21(21):19-20,2.
