中国药业2012，Vol.21Issue(22)：33-35,3.
高效液相色谱法同时测定不同产地枳壳药材中柚皮苷、橙皮苷、新橙皮苷、异柚皮苷的含量
Simultaneous Determination of Narirutin, Hesperidin, Neohesperidin and Naringin in Bittet Orange from Different Habitats by HPLC
摘要
Abstract
Objective To establish a determination method of narirutin, hesperidin, neohesperidin and naringin in Bittet Orange by HPLC. Methods The HPLC method was adopted with the Venusil XBP C18 column (250 mm×4. 6 mm,5 μm). The mobile phase was acetonitrile-0. 1% formic acid solution (gradient elution) with the flow rate of 1 mL/min. The detection wavelength was 283 nm and the column temperature was 30 ℃. Results Naringin was linear within 0. 5 - 5 μg, the correlation coefficient r = 0. 999 8, the average recovery rate was 98. 25%. Narirutin was linear within 0.08-2.01 μg.the correlation coefficient r = 0.999 8,the average recovery rate was 101. 17%. Hesperidin was linear within 0.06-1.2 μg,the correlation coefficient r = 0.999 9,the average recovery rate was 95.42%. Neohesperidin was linear within 0.09-4.82 μg,the correlation coefficient r = 0.999 7, the average recovery rate was 100.95%. Conclusion The method is simple, accurate and reliable, which is suitable for the quality control of medicinal material Bittet Orange.关键词
高效液相色谱法/枳壳/柚皮苷/异柚皮苷/橙皮苷/新橙皮苷Key words
HPLC/ Bitter Orange/ naringin/ narirutin/ hesperidin/ neohesperidin分类
医药卫生引用本文复制引用
杨华,邓义德,胡高云..高效液相色谱法同时测定不同产地枳壳药材中柚皮苷、橙皮苷、新橙皮苷、异柚皮苷的含量[J].中国药业,2012,21(22):33-35,3.基金项目
国家重点基础研究发展计划项目,项目编号:2009CB523002 （）
科技部"十二五"重大新药创制专项项目,项目编号:2011ZX09201-201-08. （）
